CONSTANTINOS PISTOS

CONSTANTINOS PISTOS

Forensic Toxicologist

  • B.S., Aristotle University of Thessaloniki, Greece
  • M.Sc., Ph.D., National and Kapodistrian University of Athens, Greece
  • Post-Doctoral: University of Athens, GA, USA

Courses Taught:

  • Toxicology lecture/lab, Chemistry and Law, Forensic Chemistry I, General Chemistry
  • Research Interests: Bioanalysis of drugs for the investigation of forensic and doping control cases.

Research Summary:

My research aims to assist the forensic toxicology labs through the development of fast, and efficient analytical methods for detecting emerging drugs in toxicological samples, facilitating thus the investigation of toxicological cases. We optimize separation and sample preparation methods using traditional and improved techniques and validate the methods for qualitative and quantitative determinations. We also conduct stability and quality control studies according to the ISO/IEC 17025. For our results we are using ELISA, liquid chromatography tandem mass spectrometry (LC-MS/MS) and HS-GC-FID. This instrumentation is also used to conduct studies involving performance enhancement drugs. We are interested in collaborating with other research groups that might be addressing research questions in toxicological analysis, quality assurance and control.

Professional Experience

  1. 2020-Present:
    Associate Professor of Toxicology, Department of Chemistry, West Chester University, PA, USA
  2. 2015-2020: 
    Assistant Professor of Toxicology, Department of Chemistry, West Chester University, PA, USA
  3. 2012-2015: 
    Assistant Professor of Toxicology, School of Medicine, National and Kapodistrian University of Athens, Greece
  4. 2006-2012: 
    Lecturer of Toxicology, School of Medicine, National and Kapodistrian University of Athens, Greece
  5. 2002-2006: 
    Laboratory director of private CRO pharmaceutical company - GLP Bioanalytical laboratory in bioequivalence studies, ILS SA, Athens, Greece
  6. 2001-2002: 
    Post-Doctoral fellow, College of Pharmacy, The University of Georgia Athens, USA

Selected Research Publications

  1. Official International Association for Therapeutic Drug Monitoring and Toxicology guideline: Development and Validation of Dried Blood Spot-based Methods for Therapeutic Drug Monitoring, Capiau S., Veenhof H., Koster R., Bergqvist Y., Boettcher M., Halmingh O., Keevil B., Koch B., Linden R., Pistos C., Stolk L., Touw D., Stove Ch., Alffenaar J-W. Ther. Drug Monit. (in press).
  2. Hydrophilic Interaction Liquid Chromatography-Electrospray Ionization Mass Spectrometry for Therapeutic Drug Monitoring of Metformin and Rosuvastatin in Human Plasma, Antonopoulos N, Machairas G, Migias G, Vonaparti A, Brakoulia V, Pistos C, Gennimata D, Panderi I, Molecules. 23(7), 1548, 2018.
  3. Bioanalytical LC-MS method for the quantification of plasma androgens and androgen glucuronides in breast cancer; E. Kalogera, C. Pistos, X. Provatopoulou, C. Christophi, G. Zografos, M. Stefanidou, Ch. Spiliopoulou, S. Athanaselis, A. Gounaris. J. Chromatogr. Sci., 54(4), 583-592, 2016.
  4. Development and validation of a LC/MS method for the determination of 9-tetrahydrocannabinol and 11-carboxy-9-tetrahydrocannabinol in the larvae of the blowfly Lucilia sericata. Forensic applications; S. Karampela, C. Pistos, K. Moraitis, V. Stoukas, I. Papoutsis, E. Zorba, M. Koupparis, Ch. Spiliopoulou, S. Athanaselis. Science & Justice, 55, 472-480, 2015.
  5. Multi-residue determination of 10 selected new psychoactive substances in wastewater samples by liquid chromatography-tandem mass spectrometry; Borova V.L., Gago-Ferrero P., Pistos C., Thomaidis N.S. Talanta, 144, 592-603, 2015.
  6. Standard addition HPLC method for the determination of a-tocopherol in adolescent swimmers; C. Pistos, M. Vlachou, V. Theiakodimitri, M. Petrou, E. Philippou, N. Middleton, I. Panderi, E. Gkikas, I. Papoutsis, Ch. Spiliopoulou, S. Athanaselis. Farmakeftiki, 26 (III), 115-122, 2014.
  7. Determination of hexane impurities in eye drops solutions utilizing HS-GC/FID; C. Pistos, S. Karampela, I. Vardakou, I. Papoutsis, Ch. Spiliopoulou, S. Athanaselis. J. Sci. and Ind. Res. 74, 461-465, 2015.
  8. Bioequivalence evaluation of two brands of glimepiride 4 mg tablets in healthy human volunteers; C. Pistos, M. Kalovidouris, C. Astraka, G. Kontopoulos and M. Koutsopoulou. Inter. J. of Clin. Pharm. and Therap., 43 (4), 203-208, 2004.
  9. Α validated liquid chromatography-tandem mass spectrometry method for the determination of mirtazapine and demethylmirtazapine in human plasma: application to a pharmacokinetic study; C. Pistos, M. Koutsopoulou, I. Panderi. Anal. Chim. Acta, 514 (1), 15-26, 2004.

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